VIJAY NEMA
SCIENTIST D

  VIJAY NEMA

Publications

Date of Joining Present Post: 01-09-2015

Address (Off):

NATIONAL AIDS RESEARCH INSTITUTE
P.B.NO.1895 :73, G BHOSARI IND ESTATE
PUNE - 411026 .
Telephone (off): 91-20-27331200
Fax : 020-271210731
Email : vnema@nariindia.org, dr.vijaynema@gmail.com
Educational Qualifications:

  • Ph.D. (Microbiology), Jiwaji University, Gwalior, India 2007
  • Post Graduate Diploma (Bioinformatics), Bioinformatics Institute of India, Noida, India. Feb 2002- 2003 (First Division)
  • M. Sc., (Applied Microbiology and Biotechnology) Dr. Hari Singh Gour University Sagar, Madhya Pradesh, India. 1999-2001, (First Division).
  • B. Sc., (Industrial Microbiology, Botany, Chemistry) 1996-1999, Dr. Hari Singh Gour University Sagar, Madhya Pradesh, India. (First Division) Higher Secondary, (10+2, Science) Army School, Sagar. 1996 (First Division)

Discipline:
CELLULAR AND MOLECULAR BIOLOGY
MICROBIOLOGY


Research Experience:
  • Trainee The Energy and Resources Institute July 2000 - Aug 2000

  • Research Scholar Dept. of Applied Microbiology and Biotechnology, Dr. H.S. Gour University Sagar Nov 2001 - Oct 2002

  • Junior Research Fellow Defense R & D Establishment Nov 2002 - Nov 2004

  • Senior Research Fellow Defense R & D Establishment Nov 2004 - Nov 2006

  • Assistant Professor College of Life Sciences, CHRI, Gwalior Nov 2006 - Apr 2007

  • Post Doctoral Fellow Institute of Life Sciences, Hyderabad Jun 2007 - Jan 2009

  • Post Doctoral Fellow Computational Structural Biology, Vanderbilt University May 2012 - Aug 2012

  • Scientist ‘C’ National AIDS Research Institute, Pune Feb 2009 - till date


    Working as Scientist ‘C’ at National AIDS Research Institute, Pune. Feb 2009 - till date

  • Molecular diagnostics of AIDS related infections.

  • Development of new diagnostic tools for AIDS related infections (bacterial, parasitic and mycobacterial infections).

  • Conservation pattern of existing and new targets of drugs in Mycobacterium tuberculosis. 

  • Metagenomic analysis of patient samples for screening and impact assessment of microbial populations using next generation sequencing. 

Post Doctoral Fellow at Computational Structural Biology, Vanderbilt University. May 2012 –Aug 2012

  • Fogarty international postdoctoral fellowship for short term training. 

  • Protein structural biology and docking.

Worked as Post Doctoral Fellow at Institute of Life Sciences, Hyderabad. Jun 2007 - Jan 2009

  • New drug discovery and vaccine development against M. tuberculosis.

  • Anticancer compound screening using various cell lines.

  • Screening antiinfective agents using various bacterial pathogens.

  • Screening newer targets for antibacterial drug design.

Assistant Professor: College of Life Sciences, CHRI, Gwalior Nov 2006 - Apr 2007 

  • Assistant Professor, Biotechnology at College of life Sciences, Cancer Hospital and Research Institute, Gwalior, Madhya Pradesh, India 

  • Taught Biotechnology to B.Sc and M.Sc students and guided a few dissertations.

Senior Research Fellow at Defence R & D Establishment: Nov 2004 - Nov 2006

  • Developed an ELISA based diagnostic system for enterotoxin B.

  • Superantigenicity alleviation of enterotoxin B through site-directed mutagenesis.

Junior Research Fellow at Defence R & D Establishment: Nov 2002 - Nov2004

  • Characterized a large number of environmental and clinical isolates of Staphylococcus aureus and Vibrio cholerae.

  • Cloning and expression of enterotoxin B gene. 

  • Optimized methods for purification of native and recombinant enterotoxin B. 

  • Generation of antibodies in laboratory animals.

Research Scholar at Dept. of Applied Microbiology and Biotechnology, Dr. H.S. Gour University Sagar: Nov 2001 to Oct 2002.

  • Screening of fungi for the isolation of species producing pharmaceutically important polyunsaturated fatty acids. 

  • Taught various topics of Applied Microbiology and Biotechnology to post-graduate students.

Trainee at TERI, The Energy and Resources Institute (Formerly: Tata Energy Research Institute): July – Aug. 2000.

  • Microbial degradation of chlorinated pesticide - Endosulfan. A bacterial consortium was developed for the degradation of endosulfan to its non-toxic components.

Courses/Trainings

  • “ICGEB-IUBMB Workshop ‘Human RNA Virus’” International Centre for Genetic Engineering and Biotechnology, New Delhi, India Feb 2010.
  • “Indo-German Workshop on Molecular Epidemiology of Infectious Diseases” University of Hyderabad, India. Nov 2008.
  • “Basic Training in Recombinant DNA Technology and PCR” Bangalore Genei, Bangalore, India. 2005.
  • “Protein Purification and Immunotechniques.” Bangalore Genei, Bangalore, India. 2005.
  • “Recent Advances in Diagnostic Virology” at DRDE Gwalior, India 2005.
  • “Recombinant DNA Technology and Molecular Detection Techniques” DRDE Gwalior, India. 2003.

Acknowledged for minor contribution:
Pattnaik et al., 2007. J. Chromatogr. B 846 184–194

Patent filed:
Process for the purification of single polypeptide chain protein, application no. 2575/DEL/2007

Sequence submissions

  • Submitted to EMBL-EBI- AM158256, 2005
  • Submitted to NCBI-GenBank- 2006: DQ997828, DQ997829, DQ997830, DQ997831, DQ997832, DQ997833, DQ997834, DQ997835, DQ997836, DQ997837, DQ535901, DQ535902, DQ630750, DQ630751, DQ630752, DQ630753, 2004: AY852244.

Papers presented in conferences

  • Vijay Nema, Ranu Agrawal, Dev Vrat Kamboj, Ajay Kumar Goel and Lokendar Singh. Food poisoning outbreak in Indian subcontinent: Isolation of heat resistant enterotoxigenic Staphylococcus aureus. In: Microbiology: The challenges ahead. 47th Annual Conference of the Association of the Microbiologists of India (AMI), 2006. pp 225. (Awarded best poster)
  • Ranu Agrawal, D.V. Kamboj, Vijay Nema, A.K. Goel and L. Singh. Development of detection system using antisera raised against over-expressed recombinant SEB. In, National Symposium on Recent Trends in Molecular Biology. Cancer Hospital and Research Institute, Gwalior, India. 2006. pp 27. (Awarded best paper)
  • Vijay Nema. Staphylococcal enterotoxin B: Gene Cloning and expression in E. coli and production of antibodies against it. In, XX M.P. Young Scientist Congress, Vikram University, Ujjain, India. 2005. pp 10.
  • Vijay Nema, Dev Vrat Kamboj, Ajay Kumar Goel and Lokendar Singh. Staphylococcal Enterotoxin B: A Comparative Evaluation of Purification Techniques. In: Microtech-2K4: 45th Annual Conference of the Association of the Microbiologists of India (AMI), 2004. pp 80.
  • Dev Vrat Kamboj, Vijay Nema, Arun Pandey, Ajay Kumar Goel and Lokendra Singh. Cloning 4 and Expression of Staphylococcal Enterotoxin B Gene and its Suitability for Antibody Generation. In, Microtech-2K4: 45th Annual Conference of the Association of the Microbiologists of India (AMI), 2004. pp 20. (Awarded best poster)
  • Vijay Nema. Indian Sweets Harbouring Enterotoxigenic Staphylococcus aureus.’ In, XIX M.P. Young Scientist Congress, Dr. H.S. Gour University, Sagar, India. 2004. pp 12.
  • Vijay Nema, D.V. Kamboj, Lokesh Nigam, A.K. Goel and Lokendra Singh. Survey of Indian foods for enterotoxigenic Staphylococcus aureus. In, National Symposium on Bioresources, Biotechnology and Bioenterprise, Osmania Unviersity, Hyderabad, India. 2003. pp 104.
Foreign Visits:
  1. 9TH INTERNATIOAL AIDS CONFERENCE (AIDS 2017) TO FRANCE
    FROM 2017-07-23 TO 2017-07-26


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