SRABANI MUKHERJEE
SCIENTIST E

  SRABANI MUKHERJEE

Publications

Date of Joining Present Post: 01-09-2012

Address (Off):

NATIONAL INSTITUTE FOR RESEARCH IN REPRODUCTIVE
HEALTH JEHANGIR MERWANJI STREET PAREL
MUMBAI - 400012 .
Telephone (off): 022-28261577
Fax : 022-24139412
Email : mukherjees@nirrh.res.in,srabanimuk@yahoo.com
Educational Qualifications:

  • B.Sc., 1985 from University of Calcutta(Presidency College) Physics, Chemistry, Physiology (Major), 1st Class
  • M.Sc., 1988 from University of Calcutta Physiology, 1st Class
  • Ph.D., 1995 from Indian Institute of Chemical Biology (CSIR),Jadavpur University Biochemistry

Discipline:
BIOCHEMISTRY
BIOLOGY
GENOMICS

Membership/Fellowship of Professional Societies/Associations :
Member of Indian Society for Study of Reproduction and Fertility (ISSRF)
Awards:
  • First award for presentation “Association of a Single Nucleotide Polymorphism in INSR Gene with Polycystic Ovary Syndrome in Lean Indian Women”. Mukherjee S, Shaikh N, Khavale S, Shinde G, Meherji P, Shah N and Anurupa Maitra. Current Opinion in PCOS and Syndrome X” organized by ICOD at Goa on 19th -21st March 2010.
  • Dr K. Bharadwaj Best Poster award for “Neonatal exposure of estradiol affects murine folliculogenesis” Shinde G, Khavale S, Nandedkar T, Mukherjee S. The International Conference on Laboratory Animal Sciences: Applications in Biomedical Research on Oct 1-3, 2009, Mumbai.
  • Fellowship for Frontier in Reproduction Training Course:Marine Biological Laboratory held at Woods Hole, Massachusetts, USA,15th May – 27th June, 2004.
  • Post Doctoral Fellowship at University of Texus Health Science
  • Centre, San Antonio, USA, 2001-2003
  • JRF and SRF by United Nations Development Project (UNDP), 1989-1995
  • Awarded JRF by University Grant Commission, India, in Net Examination in 1988
  • Ranked 2rd in the Calcutta University in M.Sc examination
  • “Parmal Bikash award by Department of Physiology, Science College,M. Sc course
  • Ranked 3rd in the University of Calcutta in B.Sc examination
  • National Merit Scholarship by University of Calcutta for B.Sc. Examination, 1985
  • National Merit Scholarship by West Bengal Board for School Leaving Examination,1980

Experienced in molecular biology, protein chemistry, genetics and flow cytometry. Our present research is mainly focused to understand the pathophysiology of an important condition of infertility namely Polycystic Ovary Syndrome (PCOS) by genetic profiling and proteomics studies. PCOS is a heterogenous, multigenic endocrinopathy and insulin resistance is one of its hallmark features. Efforts have been taken to delineate the genetic predisposition factors with emphasis on the genes involved in insulin resistance. Studies are also undergoing to identify differentially expressed proteins in human follicular fluid from women with PCOS compared to normal women, which may have functional relevance. Animal studies using a mouse model of cystic ovarian follicules is also being initiated to understand the mechanism of cystogenesis. These studies will help to understand the pathophysiology of PCOS, its genetic predisposition factors and may lead to development diagnostics approach and therapeutics against PCOS. I have studied detailed transcription mechanism using single subunit RNA polymerase of bacteriophage T7 (T7 RNAP) as a model. The primary function of RNAP is to produce RNA transcript which involves three major phases-initiation, elongation and termination. I have analyzed the changes in protein conformation and protein:nucleic acid interactions which occur during transcription initiation and termination by using a chemical nuclease tethered to cysteines introduced by site-specific mutagenesis into T7 RNAP. The membrane toxin interaction-transport of Diphtheria toxin across the cell membrane have been carried out using a photochemical approach. DNA ploidy (DNA Index- DI) and proliferative activity (S-phase fraction) of tumor cells which can be measured by flow cytometry are good indicators of prognosis. DI and S-phase fractions in different types of acute and chronic leukemias were determined and correlated with other clinical features and prognosis of the disease. Protein phosphorylation and dephophorylation mechanism plays an important role in the in parasite survival, multiplication and transformation. Protein phosphorylation and dephosphorylation mechanism has been studied in protozoan parasite Leishmania donovani. A soluble protein phosphatase has been identified and purified from the promastigote form of this parasite. Detailed characterization revealed that this is a serine/threionine phosphates and is similar to eukaryotic PP2C. The discovery of protein phosphatase along with protein kinase in the cytosolic fraction of L.donovani suggest that phosphorylation /dephosphorylation mechanism may be operative in the same manner as that in higher eukaryotic organism.

Foreign Visits:
  1. TRAINING FOR FLOW CYTOMETER FACSARIA AT BD BIOSCIENCES TRAIN ING CENTER. TO U.S.A.
    FROM 2011-11-14 TO 2011-11-18

  2. PARTICIPATION IN THE TWELTH ANNUAL SYMPOSIUM OF FRONTIERS IN REPRODUCTION. TO U.S.A.
    FROM 2009-06-11 TO 2009-06-13

  3. PARTICIPATION IN THE 5TH ANNUAL WORLD CONGRESS ON THE INSULIN RESISTANCE SYNDROME. TO U.S.A.
    FROM 2007-10-10 TO 2007-10-13


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