LAKSHMANA KUMAR YERNENI
SCIENTIST E

  LAKSHMANA KUMAR YERNENI

Publications

Date of Joining Present Post: 01-09-2012

Address (Off):

NATIONAL INSTITUTE OF PATHOLOGY
SAFDARJANG HOSPITAL CAMPUS POST BOX NO. 4909
NEW DELHI - 110029 .
Telephone (off): 91-11-26198402-8407, EXTN 411
Email : lkyerneni@yahoo.com
Educational Qualifications:

  • Ph.D Pharmaceutical Toxicology(1988).
  • NET for JRF and Lecturership, Life Sciences (1983).
  • M.Sc. Cell Biology (1983).
  • B.Sc. Honours in Zoology (1979).

Discipline:
CELLULAR AND MOLECULAR BIOLOGY



Research Experience:

Employment Details:

  • Research Officer, Institute of Pathology, New Delhi 1996-2001
  • Scientist C (Senior Research Officer), Institute of Pathology, New Delhi 2001 - 2007
  • Scientist D, (Asst. Director) Institute of Pathology, New Delhi, 2007 –2012.
  • Scientist E, National Institute of Pathology, New Delhi, 2012 to date

Field of Specialization:

  • Pigment Cell Biology:
  • Mycoplasma surveillance in Cell Culture
  • Bioengineering of skin:

Research Experience:

  • Technology development for growing cultured epidermal autograft from human
  • keratinocyte stem cells suitable for autologous grafting in burns patients.
  • Bio-Engineering of skin using a synthetic polymer as scaffold.
  • Evaluation and utilization of a guinea pig model useful for screening new therapeutic
  • regimen in vitiligo.
  • Melanoma model in C57 mice.
  • Mycoplasma contamination in Cell culture: The quality control issues.
  • Research Techniques employed:

Cell Biologyand Toxicology –

  • Cell and Tissue culture, Bioengineering of skin using scaffolding polymers
  • Colony forming assay for stem cells
  • Cell cloning
  • Microbial culture (Mycoplasma)
  • Development and use of Image analysis based quantification methods for mycoplasma
  • contamination and colony forming efficiency of stem cells
  • Karyotyping-G-banding, Histological, special staining, immuno cytological &
  • histochemical and immunofluorescent methods,
  • In Vitro quantification assays for cell proliferation by BrdU labeling, cells expressing
  • specific marker
  • In vivo assays for toxicology using mice and tumorigenesis innude mice
  • In vivo tumor (melanoma) models inC57Bl/7J mice
  • In vivo guinea pig model for vitiligo
  • In vivo mutagenesis assay using mouse bone marrow chromosomes
  • Quantification of biomolecules by spectrophotometry & HPLC-MS
  • Electron Microscopy - Scanning and Transmission
  • Affinity Chromatography, PAGE & SDS-PAGE
  • PCR & agarose gel electrophoresis, RT-PCR & Western Blot

Guidance for thesis work:

  • Supervisor for thesis work of a student for M.S. (Biomedical science) BITS, Pilani. The thesis was entitled A guinea pig model for the therapeutic evaluation of treatment  modalities in vitiligo.
  • Guide for one student awarded Ph.D (Jamia Milia Islamia 2008) with thesis entitled Development and application of a protocol for detection, prevention and elimination of Mycoplasma contamination in cell cultures.
  • Guide for one student awarded Ph.D (BITS, Plilani, 2016) with thesis entitled A study on differential growth-arrest of 3T3 Fibroblasts used as Feeders for Stem cell Propagation.
  • One student with the proposed research title of Evaluation of epithelial-mesenchymal interactions using differentially growth-arrested Feeder cells to cultivate human epidermal keratinocytes is currently registered for Ph.D with Jamia Hamdard University, New Delhi
Membership/Fellowship of Professional Societies/Associations :
International Pigment Cell Society
Awards:

1. Received Shri Shyam Lal Saksena Memorial Award (2006) in the field of BioMedical Engineering by the NAMS [National Academy of Medical Sciences (India)]. The award was presented at the 46th Annual Convocation of NAMS, held at National Institute of Nutrition, Hyderabad, on 28th October 2006.

2. Awarded WHO In-country Fellowship in the field of study of Research Methodology at the National Institute of Cholera and Enteric Diseases, Kolkata between 8th and 21st November 2004.

Teaching Experience:

1. Teaching faculty for M.S. (Biomedical Sciences), an integrated PG-Ph.D course for 5 academic years.

2. Teaching faculty: Undertook a course on Cell and Tissue culture- Course No. BIO G641 for the off campus pre-PhD program of BITS, Pilani, 2003 –to date.

3. Teaching Faculty: Refresher Course in Bio-Science for the faculty members of different Colleges and Universities conducted at the Academic Staff College, Jamia Milia Islamia,

2005-06.

4. Teaching Faculty Training Course for Pathologists and basic & Advanced level Training Course for technicians supported by WHO and Ministry of Health and Family Welfare

2004- to date.

Guidance for thesis work:

Masters: Four students Completed:

1. Supervisor for thesis work of a student for M.S. (Biomedical science) BITS, Pilani. (2003) The thesis was entitled A guinea pig model for the therapeutic evaluation of treatment modalities in vitiligo.

2. Supervisor for thesis work of a student for M.Sc (Biotechnology). Allahabad Agricultural Institute-Deemed University, Allahabad, (2006). The thesis was entitled Ideal conditions for the attenuation of 3T3 fibroblast feeder layer cells suitable for stem cell culture.

3. Supervisor for thesis work of a student for M.Sc (Microbiology), Jiwaji University, Gwalior (2006). The thesis was entitled Optimal attenuation conditions for 3T3 fibroblast feeders suitable for use in stem cell culture

4. Supervisor for thesis work of a student for M.Sc (Biotechnology), School Of Biosciences, Suresh Gyan Vihar University, Jaipur (2011). Evaluation of Ciprofloxacin as a routine cell culture antibiotic compared to standard penicillin & streptomycin combination

Ph.D: students (Completed):

1. Dr.Askhik Kumar awarded Ph.D with thesis entitled Development andapplication of a protocol for detection, prevention and elimination of Mycoplasma contamination in cell

cultures from Jamia Milia Islamia, New Delhi

2. Dr.RishiMan Chugh awareded Ph.D with thesis entitled A study on differential growtharrest of 3T3 Fibroblasts used as Feeders for Stem cell Propagation from BITS, Plilani.

Ph.D: Students (currently registered):

1. Mr.Madhusudan Chaturvedi is registered with Jamia Hamdard with the proposed research title of Studies on Epithelial-Mesenchymal interactions using human epidermal

keratinocytes and growth arrested fibroblast feeders.

2. Mr.Amal Dev with proposed research title of A study on non-xenogeneic approach for growing cultured epidermis seeking Ph.D registration with Indraprasta University.

Extra Mural Projects:

1. Project Title In vitro cultivation of differentiated epidermis from human keratinocytes suitable for autologous grafting in burns patients

Subject NCD

Area Ad hoc project

Start Date 2000-10-01

End Date 2004-09-30

Status Completed

2. Project Title Cell culture contamination with Mycoplasma in basic and applied biomedical research

Project Number 2004-03570

Subject Cellular and molecular biology

Area Fellowship

Start Date 2005-09-01

End Date 2008-08-31

Status Completed

3. Project Title Investigation into the utility of a patented synthetic thermo-reversible hydrogel polymer as supportive matrix towards the development of 3-D composite skin for application in wound healing and other dermatological disorders

Project Number 2006-06400

Subject Stem cell research

Area Ad hoc project

Start Date 2007-06-20

End Date 2010-06-19

Status Completed

4. Project Title A novel arithmetic approach for fool-proof production of growth arrest in 3T3 cells suitable for human epidermal culture

Project Number 2009-02800

Subject Cellular and molecular biology

Area Ad hoc project

Start Date 2010-06-21

End Date 2012-06-20

Status Completed

5. Project Title Studies on epithelial- mesenchymal interactions using human epidermal keratinocyte stem cells and innovatively growth-arrested fibroblast feeders in 3-D collagen model

Project Number 2010-05750

Subject Stem cell research

Area Fellowship

Start Date 2010-11-10

End Date 2013-11-09

Status

Ongoing

6. Project Title Novel growth arrest protocol for human dermal fibroblasts useful as non-xenogeneic feeders for clinically applicable epidermal

Project Number culture2012-15350

Subject Stem cell research

Area Ad hoc project

Start Date Approved

End Date Financial sanction awaited

 

Foreign Visits:
  1. PARTICIPATION IN THE 16TH INTERNATIONAL CONGRESS INTERNATIONAL ORGANIZATION FOR MYCOPLASMOLOGY. TO UNITED KINGDOM
    FROM 2006-06-09 TO 2006-06-14

  2. PARTICIPATION IN THE XVIII INTERNATIONAL PIGMENT CELL CONFERENCE (PICC). TO THE NETHERLANDS
    FROM 2002-09-09 TO 2002-09-13


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