NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES (NICED) ANNUAL REPORT 2004-2005

 

Particulars

Page No.

Preface

1

1. Study On Hospital Surveillance 

14

1.1. Hospital based surveillance system for diarrhoeal diseases

14

2. Community Based Studies 

15

2.1. Acute lower respiratory tract infection (ALRI) and diarrhoea in rural children below two years in relation to feeding practices with particular reference to breast feeding : A community based study

15

2.2 . An operational study on Effect of zinc supplementation on reduction of diarrhoeal morbidity amongst rural children. (In collaboration with Health Department, Government of West Bengal)  

15

2.3. Epidemiology of typhoid fever in a rural and urban slum community of West Bengal  

19

2.4 Identifying environmental risk factors for endemic diarrhoeal diseases in West Bengal, India: a remote sens-ing- geographic information system(GIS) approach  

21

3. Clinical Studies

22

 3.1. Evaluation of comparative efficacy of Cefuroxime axetil and Tetracycline in the treatment of cholera in adults

22

3.2. Impact of Lactobacillus (Lactic acid bacteria) in children with acute watery diarrhoea  

22

4. Studies on Vibrio cholerae

25

4.1. Studies on the structure-function relationship of Vibrio cholerae hemolysin (HlyA)  

25

4.2. Sequential studies of Vibrio cholerae 0139 and some of its defined virulence phenotypes on intestinal pathology in rabbit ileal loop model  

26

4.3 Vibriocidal assay  on rough vibro cholerae strains with acute and convalescent sera collected from cholera patients and implication of gene(s) for the rough phenotype

26

4.4. Spread of cholera with vibro clolerae 01,Serotype Inaba in India.  

27

5. Studies on Vibrio cholerae Phages

30

5.1. Further application of phage typing scheme for discriminating strains of Vibrio cholerae O139 

30

5.2. Nationwide screening of phage types of V. cholerae O1 biotype EltTor

30

5.3. Molecular analysis of Vibrio cholerae bacteriophages: cloning and sequencing of phage DNA  

30

6. Studies on Shigella species

36

6.1. Molecular characterization of multi-drug resistant Shigella flexneri in Kolkata 

36

6.2. Up-regulation of mucosal immune response by porin of Shigella dysenteriae type 1: Study of Toll-like receptor, costimulatory molecule and immunoglobulin expression on murine B-1 cell  

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6.3. Antigenic recognition of Shigella dysenteriae outer membrane proteins using human convalescent sera and to evaluate their role in cell-mediated immune response in shigellosis 

38

7. Studies on Escherichia coli

39

7.1.Studies on  the binding of Escherichia coli.....

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7.2. Virulence Characteristics and molecular Epidemiology........

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7.3. Electron microscopic study of ultra structures associated with Intestinal cell adherence of enteroaggregative Escherichia coliisolated from acute hospitalized diarrhea cases and healthycontrol children from Kolkata 63  

43

7.4. Developed of a  PCR  based  assay for detection of .......

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8. Studies on Helicobacter pylori

47

8.1. Correlation of histology with genotypes of Helicobacterpylori isolated.......

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8.2. Most Helicobacter pylori starins of Kolkata are resistant to ......

48

9. Studies on other bacterial pathogen

51

9.1. Mode of action of Yersinia enterocolitica heat stable enterotoxin (YSTa) in rat intestinal epithelial cell

51

10. Studies on Diarrhoeagenic Parasites

52

10.1. Studies on rRNA processing in Giardia lamblia

52

10.2. Studies on electron dense granules of Entamoeba histolytica containing collagenase activity

54

11. Studies on Viral Pathogens

61

11.1. Genotyping of Group a rotavirus strains:

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11.2. Studies on astrovirus causing acute watery diarrhoea among chidren in kolkata ,India

62

11.3. Studies on human caliciviruses by Reverse Transcripti on-Polymerase Chain Reaction [RT-PCR] to detect the presence of Norwalk like virus [NLV] and Sapporo like virus [SLV]

63

12. Studies on HIV/AIDS

68

12.1. Study on sexually transmitted infections among female sex workers of West Bengal

68

13. Epidemic Investigations

74

13.1. Investigation of acute diarrhoeal dieses at Tripura

74

13.2.  Investigation  diarrhoeal dieses out break in delhi  Tripura 

76

13.3. Investigation   of out break ...

76

13.4. Testing of stool and drinking water samples received from other parts of West Bengal

76

13.5.  Epidemic Investigation of diarrhoeal  out break in Garulia, North 24 Parganas

77

13.6. diarrhoeal  dieses  out break in different words of Behala ,Kolkata ,West Bengal

80

14. Activities of National HIV Reference Centre of NICED, Kolkata

94

14.1 Phylogenetic analysis of HIV-1 95
15. NICED-JICA Project

100

15.1 Summary of Activity of the project in FY 2004-05  and Activity of the project

100

16. IVI-NICED Collaborative Project

108

16.1. Surveillance for typhoid fever and cholera in eastern Kolkata, West Bengal, India

108

17. Other Extramural Project

113

17.1. Hib Vaccine Probe Study -Part A

113

17.2. Rotavirous Genomics

114

17.3. A Multicentric Study On Surveillance And Molecular Characterization Of Human Caliciviruses(HuCVs) from Cases Of Acute Gastroenteritis

115

17.4. Genogrouping rotaviruses of human and animal origin in india

115

17.5. Porin of Shigella dysenteriae Mediated regulation of tool -like receptor and Costimulatory...

116

17.6. Study of the mechanism of transformation of vibro cholerae hemolysin...

116

17.7. Molecular vAspects of a 47-kDa  N-acetyl-D- Glucosamine specific Vibrio cholerae Adhesin

117

17.8. Phase II and Phase III human trails with candidate oral vacine VA1.3-a multicentric study

117

17.9. Detection ,distribution and functional analysis of CTX prophages of toxigenic non -01, non-0139 vibrio......

118

17.10. Estimation of HIV load among  different  risk groups of West Bengal

118

18.  Activities of the Training and Extension Division

120

18.1.Two training programmes for German Doctors on tropical and travel medicine jointly organized......

120

18.2 National Technology  day calebration on may 11,2004

120

18.3. ph.D. orientation course from 14-18 June ,2004:

120

18.4 Training programme on Clinical managment and Diagnostic.../18.5. visit Mr Shogo Arai...

120

18.6. Four Extramural Training programmes on Immunization ......./18.7. WHO Biennium funded workshop on Data Based Generation on Disease Burden Estimation 121
18.8 Workshop of Immunology Study Group West Bengal/18.9. Training programme for the medical students of japan 122
18.10. Training promme of INFORMATICS/18.11 Organize the visit of students of Chaitanya PG College Andhra Pradesh 123
18.12. JICA-NICED Domestic Training programme on Molecular...../18.13. Training programme on Molecular Genetics of vibro...... 123
18.14 3rd Country  JICA-NICED Domestic Training programme on Molecular Epidemiology of ......... 123
18.15 Training programme for WHO fellows for Research Methadology/18.16. Training programme for WHO fellows for ..... 125
18.17 Training programme for Clinical Manajment of Acute Diarrhoea /18.18. Training programme for International WHO fellows for .... 126
18.19 Training programme for delhi...../ 18.20. Training programme for kolkata..... 126
18.21 ICMR-Lancet -IPHA (WB) workshop on medical paper writing/18.22. 3rd phase External Quality Assurance Scheme for HIV testing funded by NACO / 18.23 National Science Day celebration 126
18.24 All india meeting and workshop on phase III......./18.25. Training programme for the medical students of Japan/18.26. Organize the lecture of Guest Speakers 127
18.27 Summer Training programme ..../18.28. Organise the Institutionalseminars for the Scientsts and fellows 128

19. Scientific Conference/Seminar/Meeting Attended By The Scientists/Research Fellows/Staff

129

20. Training/Awards received by the Scientists/Fellows/Staff

170

21. Other activities of the Scientists

143

22. List of Publications

146

23. Committees of the Institute

151

24. List of the Staff

155